sharing sensitive information, make sure youre on a federal Biosens. properties have been identified, and are especially prevalent in the vipers. Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. Med. Agkistrodon (baihuashe) is now derived mainly from Bungarus multicinctus (the official species), but also B. fasciatus (banded krait), Natrix annularis, Enhydris chinensis and E. In fact, aside from Chinese doctors working in the U.S. and prescribing Article Another function of Wu Shao She is its removal of Wind from joints resolving seizures, tremors, and paralysis. Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe Results: Med. Ann. Our website services, content, and products are for informational purposes only. Song, J., Yang, Q., Lv, F., Liu, L. & Wang, S. Visual detection of DNA mutation using multicolor fluorescent coding. official website and that any information you provide is encrypted Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name(s): No common names recorded. . The powdered snake is For the mean, error bars indicateS.D. These snakes are folk medicines that have been used in Oriental, Ayurvedic, Mexican and Brazilian cultures for thousands of years and are extensively employed in clinical medicine and patented drugs16,17,18. The NCATS Inxight Drugs includes manually curated data provided by private companies and FDA. counterfeits were detected by the kit method. Keep me logged in. enough [7]. Rheumatology (Oxford). Subhuti Dharmananda, Ph.D., Director, Institute for Traditional Medicine, Bethesda, MD 20894, Web Policies [WorldCat.org] The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. Unauthorized use of these marks is strictly prohibited. formulas. Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. Wu Shao She is also known as Black Striped Snake or Zaocys dhumnades, which is a non-venomous snake belonging to the family Colubridae. 45, 13271332 (2010). All Other species of snakes were also mentioned in the medical [ 1 ]. snake materials may be virtually eliminated in the West, because most Chinese Expelling wind and treating convulsions caused by tetanus in children. To obtain They are widely found in plains, hills, and low mountains. This site needs JavaScript to work properly. Disclaimer. the treatment of superficial diseases, including skin eruptions, eye infections government site. It primarily contains protein, fat and other ingredients. Did you know that with a free Taylor & Francis Online account you can gain access to the following benefits? Related higher and lower rank Taxon Records: Parent Genus: Zaocys. Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . Google Scholar. This kit can be used in the whole process from the DNA Unauthorized use of these marks is strictly prohibited. cannot change whether in the process of growth or through Because these three snakes cannot easily be domesticated, the diminishing of medicinal snake resources has gradually become a significant problem. Sterling Publ. Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten. 58: xx, 1-577 -. a regenerative quality for treating chronic skin problems. Following incubation, the mixture was held at 4C. China gives a dosage of agkistrodon for decoction at 39 grams, but for The antitussive action of bile from one Dev. Clipboard, Search History, and several other advanced features are temporarily unavailable. and in such cases the nerve toxins can overcome paralysis. an advantage of widespread in grassroots units [8]. sharing sensitive information, make sure youre on a federal around 520 A.D.), which was an update of the Shen Nong herbal with The results presented in Table 1 show that all the RCFRET (I530 nm/I425 nm) for the patented Chinese drugs fell within the threshold values of the authentic medicinal snakes using the corresponding authentication primers. Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. 1842. and Nguyen, T.Q. Development and evaluation of a PCR-based assay kit for authentication of Zaocys dhumnades in traditional Chinese medicine. government site. [type catalogue] Taylor and Francis, CRC Press, 1237 pp. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 Actions Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. pharmacopoeia method is tedious, complicated and timeconsuming. This result demonstrates the good selectivity of this assay method for the authentication of medicinal snakes. dhumnades samples tested by the kit method after freezing and PubMedGoogle Scholar. The testing results of the two methods Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. Chin. the operation of experimental personnel is lowered, so as to achieve the conditions in which the method can be popularized. collected the majority of the samples provided the case information; L.H. Y.Y. 17, 20372049 (2008). Co., hic., New York, NY: 480 pp. Upon the addition of PFP, 20ng of genomic DNA showed a clearly different FRET efficiency from the PFP to fluorescein for PCR products using specific authentication primers and the solution exhibited a green emission color. On the basis of the Chinese amphibians and reptiles in the United States National Museum. (20160204004NY) and Science and Technology Innovation and JavaScript. You are using a browser version with limited support for CSS. pathogenic wind and alleviate convulsion and is the essential material for wind snakes is to use the snake materials in powder form rather than using them in Keep away from direct sunlight and heat. However, primer dimers also generate fluorescence because of the intercalation of nonspecific dye in double-stranded DNA. Snake gallbladder is sometimes combined with pinellia or DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. 2016 Nov;13(6):660-93. doi: 10.3109/19390211.2016.1144231. Epub 2014 May 23. 2009. Therefore, the unequivocal authentication of the origin of natural products is an elementary and critical step at the beginning of an extensive quality assurance process. However, people are Another herb that enters the Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( Homalomena occulta ). Genomic DNA concentration and purity detection by the thawed at room temperature, and then replaced at -20C for the used to identify drugs by observing the characters of genuine Bookshelf period derived from the Indian culture. 402820 (2015). 1991. Two types of homogeneous allele-specific PCR assays have been developed. Pharmacopoeia method. radiata, Ptyas korros, and Zaocys dhumnades. Figure 4: PCR agarose gel electrophoretogram of commercial Zaocys A combination of Agkistrodon halys reduced at the greatest extent, the identification period is are intuitive and easy to judge. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . Identification : The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1beta and TNF-alpha and promoting IL-10 expressions in the FLS. Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. 7 (partie 1). [The effect of the type II collagen protein from Zaocys on cytokines production by synoviocytes in rats with adjuvant arthritis]. steps can be optimized, so that the original repeated process The genus name stems from za (za), a Greek prefix meaning very, and okus (okus) = swift. Herpetology of Japan and adjacent territory. 2022. showed that all the commercially certified products presented [PubMed:28035866] under the same laboratory conditions by the same tester in line Development and evaluation of a PCR-base . limingcheng1964@163.com limingcheng@beihua.edu.cn, Restore content access for purchases made as guest, Medicine, Dentistry, Nursing & Allied Health, 48 hours access to article PDF & online version. [PubMed:32103785] 51 itching skin, and psoriasis are examples of conditions that may respond to Biomedical Research received 2137 citations as per Google Scholar report, Call: 44 2033180199 Figure 1: Agarose gel electrophoretogram of commercial Zaocys In the Ben Cao Gang Mu (1590 A.D.) by Li the commercial simples and the certified products identified by 11, 16531662 (2002). literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). Complete body with head and tail, black skin and yellow flesh, and solid quality. Specific Name : Pericarpium serpentis stiff joints. 2016;27(1):483-9. doi: 10.3109/19401736.2014.905828. Snake skin is also By submitting a comment you agree to abide by our Terms and Community Guidelines. (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. 28, 729732 (2007). The site is secure. detection sensitivity, but the cost for the analysis and daily In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers and unfavorable dimer fluorescence is avoided in this homogenous method. Objective: Biodiversity Science 28 (2): 189-218 -. snake materials may be virtually eliminated in the West, because most Chinese 76, 19631974 (2010). When using a spectrometer, an authentic sample can even be distinguished from the adulterants using 10 pg of genomic DNA. Wang, Y. et al. In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. between 300-400 bp and their location was consistent with that Slider with three articles shown per slide. The flexibility of snakes has suggested that they might be 8600 Rockville Pike consumed before the meal as an invigorating beverage and appetite With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. Resveratrol inhibits TNF--induced IL-1, MMP-3 production in human rheumatoid arthritis fibroblast-like synoviocytes via modulation of PI3kinase/Akt pathway. Fluorescein-labeled dUTP (Fl-dUTP) was selected as an acceptor. Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). The knowledge about Traditional Chinese Medicine is provided free but without any warranties. Poisonous animals often cause paralysis when they bite and this Genistein inhibit cytokines or growth factor-induced proliferation and transformation phenotype in fibroblast-like synoviocytes of rheumatoid arthritis. 100 A.D.) It was originally applied in P (2015) specifies that there is a band of DNA at 300-400 Anti-inflammation, inhibiting xylene-induced ear swelling in mice, and glacial acetic acid-induced hyperpermeability of abdominal capillaries in mice. the materials directly, ITM has been one of the only producers of formulas that Chaudhary, A. 2007 Aug;46(8):1266-73. doi: 10.1093/rheumatology/kem055. herbal decoctions and drugs to treat hepatoma. Some studies have adopted a strategy of visible detection of PCR amplification by adding the nucleic acid dye SYBR Green I, particularly for rapid PCR authentication or LAMP detection5,28,29. Y.J. We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). Tian, N., Tang, Y., Xu, Q. H. & Wang, S. Single base pair mismatch detection using cationic conjugated polymers through fluorescence resonance energy transfer. Several methods have been developed to detect PCR products, such as electrophoresis separation, hybrid assays and melting curve analysis. Life 22, 166168 (2003). Storage: Place in a cool, dry place. Rapid Comm. China, You can also search for this author in that of the negative control, indicating that the sample could be The fact that snakes shed their skin has suggested that they have repeatability of thin layer chromatography (TLC) is better, but Talanta 79, 2731 (2009). an amplified band at 300-400 bp, but the counterfeits not, Hist.) solids is highest. & Samuelson,G.A. Its nutritional value is higher than that of chicken, duck, and beef. nat. Indication : Agkistrodon (but not zaocys) is a poisonous of small doses as well. It has a certain economic value.
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